Aqueous biphasic systems based on Pluronics: An overview of the last 10 years

Abstract

This review explores the significant advancements achieved with the use of Pluronic® triblock copolymers in aqueous biphasic systems (ABS) for biomolecule extraction and processing. Pluronic-based ABS, characterized by their superior stability and selectivity, have emerged as a promising alternative to traditional ABS. Harnessing the unique amphiphilic nature of Pluronic copolymers, these systems enable stable liquid–liquid phase separation, making them highly suitable for the selective extraction of diverse biomolecules. The review highlights several advantages of Pluronic-based ABS, including their versatility based on its PEO<inf>X</inf>PPO<inf>Y</inf>PEO<inf>X</inf> (ABA) structure, enhanced partitioning efficiency, selective extraction, low shear stress, and structural stability. Additionally, they offer excellent solute recovery and the potential for biomolecule encapsulation within Pluronic micelles. Although high costs and complex formulations present challenges, the remarkable benefits position Pluronic-based ABS as invaluable tools in biomolecular research and across the biotechnological, pharmaceutical, and biomedical industries. This review provides in-depth insights into recent advancements and the growing potential of Pluronic-based ABS in the evolving fields of biomolecule extraction, purification, and nanoencapsulation. © 2025 Elsevier B.V., All rights reserved.