Browsing by Author "Sanchez-Gonzales, Gloria"

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Compuestos fenólicos y actividad antioxidante de los extractos de la hoja de chirimoya (Annona cherimola Mill)
(Universidad Nacional de Colombia, 2019-5-1) Sanchez-Gonzales, Gloria; Castro-Rumiche, Carlos; Alvarez-Guzman, Gary; Flores-Garcia, Jorge; Barriga-Sanchez, Maritza
Después de la cosecha del fruto chirimoya, los árboles liberan enormes cantidades de hojas, las cuales son quemadas por los agricultores. Este trabajo muestra que las hojas pueden ser usadas para nuestro beneficio como antioxidante. Se determinaron los compuestos fenólicos (CF) y la actividad antioxidante (AA) de los extractos de la hoja seca de Annona cherimola Mill en etanol al 70% v/v, agua a 80 °C, y agua subcrítica (AS) a 110, 120 y 130 °C, siguiendo un diseño factorial con el programa Minitab. Los CF se cuantificaron con la metodología de Folin Ciocalteu y la AA con el 2,2-difenil-1-picrilhidrazilo (DPPH) y con el poder antioxidante reductor del hierro (FRAP). Los resultados indicaron que el tipo de solvente y el tiempo de extracción presentaron un efecto significativo en el contenido de CF y AA de los extractos. Se concluyó que el extracto de hoja de chirimoya es un potencial antioxidante. El extracto de AS a 130 °C presentó el mayor contenido de CF (5,6 g EAG/100 g de hoja seca) y el extracto etanólico presentó mayor AA (0,86 mg equivalente trolox/mg extracto seco; IC50=0,020 mg de extracto seco/mL de extracto de hoja seca y FRAP de 1710,14 μmol equivalente trolox /g de hoja seca) y los extractos obtenidos con AS a menor temperatura presentaron mayores valores de AA.
Estudio preliminar de teñido de fibra de alpaca utilizando extracto acuoso de molle (Schinus molle) como pigmento natural
(Instituto Tecnológico de la Producción, 2022-01-21) Carrasco Bocangel, José; Sucasaca, Alexander; Sanchez-Gonzales, Gloria; Morán Flores, Geraldyne; Barriga-Sánchez, Maritza
"El teñido de prendas con pigmentos naturales es una alternativa para evitar utilizar colorantes químicos que a menudo resultan ser tóxicos para el medio ambiente y la salud humana. El objetivo del estudio fue realizar el proceso de teñido de la fibra de alpaca con pigmento natural a partir de molle fresco y seco. Para ello, se realizó la extracción líquida presurizada acuosa del pigmento de molle, se midió el color (L*, a* y b*), pH, sólidos totales y se evaluó la estabilidad del extracto en almacenamiento. Se realizó la evaporación parcial del agua y con el extracto concentrado se realizó el teñido de la fibra de alpaca utilizando los siguientes mordientes: ácido cítrico, sulfato de cobre, sulfato de hierro, sulfato de aluminio, bitartrato de potasio, sulfato de amonio, evaluándose la intensidad del color, así como la solidez de color al frote y al lavado. Los resultados indicaron que la mejor condición de almacenamiento del extracto fue 5°C en oscuridad y que la evaluación de solidez del color al frote como al lavado de las madejas mostraron valores de 4-5 utilizando los mordientes mencionados, aunque con el ácido cítrico se realizaron menos enjuagues. Se concluye que el ácido cítrico fue el mordiente con mejor afinidad al molle, y que para el teñido de fibra de alpaca fueron necesarios 21 y 4 ml de extracto concentrado de molle fresco y seco respectivamente fue satisfactorio."
Functional characteristics and antimicrobial activity of supercritical CO2 extracts from passion fruit (Passiflora edulis) seeds
(Universidad Nacional de Colombia, 2025-1-1) Barriga-Sánchez, Maritza; Varas Condori, Miguel Angel; Sanchez-Gonzales, Gloria; Céspedes Chombo, Roxana
The passion fruit seed is an important byproduct of the agroindustry, as it accounts for 15% of this fruit, so it is imperative to add value to these seeds. In this context, the present study aimed to evaluate the functional characteristics and antimicrobial activity of passion fruit seeds. Oil extraction was carried out using supercritical CO2 (SC-CO2) and supercritical CO2 with ethanol cosolvent (SCCO2+et); both oils showed a similar fatty acid profile with a high content of polyunsaturated fatty acids (71.62 and 71.80%, respectively). The study of the functional quality of the oils showed low atherogenicity (AI) and thrombogenicity (TI) indices. The seed flour oil and the defatted passion fruit seed flour extract presented antimicrobial activity against Klebsiella oxytoca, Staphylococcus aureus and Proteus vulgaris. The ethanolic extract of flour defatted with CO2+ethanol (FDCE) obtained higher values of total phenolic compounds and antioxidant capacity by ABTS and FRAP. The present research provides the characterization of the functional properties of the passion fruit seed oils and the defatted seed, being data of interest for future applications of the passion fruit seed.
In vitro and in vivo evaluation of hypoglycemic potential and acute toxicity of microencapsulated turmeric instant powder
(Phytomedicine Plus, 2024-11-01) Barriga-Sánchez, Maritza; González-Aspajo, Germán; Sanchez-Gonzales, Gloria; Barreto-Salcedo, Candy; Aranda-Ventura, Jose Alberto
Background: Curcuma longa L. (turmeric), traditionally used to treat various ailments including diabetes, has demonstrated beneficial health effects. However, its potential in food formulations requires further investigation. Purpose: This study examined the hypoglycemic effects of an instant powder containing microencapsulated turmeric oleoresin both in vitro and in vivo, alongside an acute toxicity assessment in Holtzman rats. Methods: This study encompassed both in vitro and in vivo evaluations. In vitro, the α-glucosidase (a digestive enzyme) inhibition assay was employed to determine the powder's effectiveness. In vivo assessments included: (1) evaluation of the hypoglycemic effect in mice; (2) conducted safety testing following international guidelines, administering a 2000 mg/kg dose to rats with subsequent monitoring of biochemical parameters; and (3) histological examination of liver, kidney, and spleen tissues for potential harmful effects. Results: The instant powder with turmeric oleoresin microencapsulation demonstrated significant α-glucosidase inhibition in vitro and hypoglycemic effects in vivo. The acute toxicity test at 2000 mg/kg showed no mortality but revealed significant alterations in erythrocyte parameters and hemoglobin percentage. Histological examinations of liver, kidney, and spleen tissues did not differ from those of the control group, though pancreatic tissue was not evaluated in this study. Conclusion According to this study, the microencapsulated turmeric oleoresin instant powder exhibited antidiabetic effects without signs of overt toxicity at doses up to 2000 mg/kg in rats.
Optimization of the extraction of Curcuma longa L. oleoresin with supercritical CO2. Evaluation of antioxidant and hypoglycemic activity.
(Academia Brasileira de Ciências, 2025) Barriga-Sanchez, Maritza; Cueva -Martinez, Miguel; Sanchez-Gonzales, Gloria; Gonzalez-Aspajo, German; Villacres-Vallejo, Jorge
The fresh rhizomes and flour of turmeric (Curcuma longa L.) have been used for centuries in traditional medicine to treat ailments. In recent years, attention has been paid to the beneficial properties of turmeric such as the prevention and control of diabetes. The objectives of this study were to optimize the extraction parameters of turmeric oleoresin (TO) with supercritical CO2 and ethanol (SC-CO2), and to evaluate the antioxidant activity and hypoglycemic activity in vitro and in vivo. The results showed that the optimal conditions for oleoresin extraction using SC-CO2 were 188 bar, 65°C and 180 min; the yield obtained under optimal conditions was 16.09 ± 0.57 oleoresin/100 g turmeric flour; antioxidant activity (ABTS) was 2224.96±26.33 μmol TE/g oleoresin; the mean inhibitory concentration of α-glucosidase (IC50) was 11.59 ± 2.05 μg/mL; and the greatest hypoglycemic effect in vivo in mice was obtained at the concentration of 200 mg of TO /kg body weight. This work shows the optimized conditions to extract the greatest amount of TO with the highest content of curcuminoids and antioxidant activity, and the in vitro and in vivo hypoglycemic effect of TO, for its possible use in functional foods.