Browsing by Author "Llanos-Cabanillas, Luis Armando"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Estudio de adaptación del cultivo de piña (Ananas comusus L.) variedad MD 2 cv. Golden bajo tres dosis de fertilización y tres densidades de siembra con riego por goteo en suelos arenosos de Piura
    (ITP red CITE, 2022-08-10) Chinchay-Huaman, Luis Fernando; Guzman-Farfan, Luis Conrado; Robles-Cueva, Henry; Llanos-Cabanillas, Luis Armando; Arbulu-Zuazo, Arturo Adolfo
    Esta investigación se realizó entre julio 2019 y junio 2020 en el Valle de Cieneguillo sur, margen derecha a 29 m. s. n. m., con el fin de buscar adaptar un cultivo de piña de clima tropical, suelo ácido y temperatura máxima de 30°C y 15°C de mínima y precipitaciones de hasta 2000 mm (Selva Central), a un terreno arenoso, básico, con temperatura máxima de 39°C y 14°C de mínima, con precipitaciones de hasta 103.2 mm o con "Niño" alrededor de 2148 mm, logrando productividad y rentabilidad que lo haga viable, para la demanda local de consumo fresco y de "fábrica" para las empresas transformadoras. Los objetivos fueron evaluar la fenología del cultivo de piña con tres dosis de fertilización y tres diferentes densidades de siembra, dividiendo las pruebas en 1 ha, determinar la combinación que influya mejor en el crecimiento, desarrollo del cultivo y los frutos en la cosecha, en un terreno arenoso. Para el ensayo en campo se analizaron variables de producción del fruto por tratamiento, rendimiento por unidad experimental, grados Brix y porcentaje de acidez en fruto. También la variable de análisis foliar para observar la extracción de nutrientes y el rendimiento económico de los tratamientos empleando riego tecnificado y fertilización de macro nutrientes foliar. En la interacción de los tratamientos en estudio, las combinaciones D?T?, D?T? y D?T? presentaron los mejores resultados en la relación B/C, con S/1.58, S/1.36 y S/1.13 respectivamente.
  • Thumbnail Image
    Item
    Innate and adaptive immune response of Rainbow trout (Oncorhynchus mykiss) naturally infected with Yersinia ruckeri
    (Elsevier BV, 2024-08) Soto-Dávila, Manuel; Rodríguez-Cornejo, Tania; Benito, Valentina Wong; Rodríguez-Ramos, Tania; Mahoney, Gracen; Supinski, Rochelle; Heath, George; Dang, Xiaoqing; Valle, Fernando Mesías; Hurtado, Carmen; Llanos-Cabanillas, Luis Armando; Serrano-Martínez, Enrique; Dixon, Brian
    Rainbow trout is an important fish species for Peruvian artisanal aquaculture, comprising over 60 % of the total aquaculture production. However, their industry has been highly affected by several bacterial agents such as Yersinia ruckeri. This pathogen is the causative agent of Enteric Redmouth Disease, and causes high mortality in fingerlings and chronic infection in adult rainbow trout. To date, the immune response of rainbow trout against Y. ruckeri has been well studied in laboratory-controlled infection studies (i. e. intraperitoneal infection, bath immersion), however, the immune response during natural infection has not been explored. To address this, in this study, 35 clinically healthy O. mykiss without evidence of lesions or changes in behavior and 32 rainbow trout naturally infected by Y. ruckeri, were collected from semi-intensive fish farms located in the Central Highlands of Peru. To evaluate the effect on the immune response, RT-qPCR, western blotting, and ELISA were conducted using head kidney, spleen, and skin tissues to evaluate the relative gene expression and protein levels. Our results show a significant increase in the expression of the pro-inflammatory cytokines il1b, tnfa, and il6, as well as ifng in all three tissues, as well as increases in IL-1β and IFN-γ protein levels. The endogenous pathway of antigen presentation showed to play a key role in defense against Y. ruckeri, due to the upregulation of mhc-I, tapasin, and b2m transcripts, and the significant increase of Tapasin protein levels in infected rainbow trout. None of the genes associated with the exogenous pathway of antigen presentation showed a significant increase in infected fish, suggesting that this pathway is not involved in the response against this intracellular pathogen. Finally, the transcripts of immunoglobulins IgM and IgT did not show a modulation, nor were the protein levels evaluated in this study. © 2024 Elsevier B. V., All rights reserved.