Browsing by Author "Cabrera-Soregui, Milagros"

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    Draft genome of the multidrug-resistant Citrobacter freundii strain CAPA023 isolated from Arapaima gigas in Peru: Is it a reservoir of resistance and virulence genes?
    (Elsevier BV, 2025-5) Garcia-Candela, Enrique; Mondragón-Martínez, Aaron; Noceda-Rodríguez, Manuel; Mesias Valle, Fernando; Cabrera-Soregui, Milagros; Valverde-Vera, Veronica; Benito-García, Maria; Romayna-Ríos, Romina; Verástegui-Tello, Miriam; Dubey, Saurabh; Munang'Andu, Hetron M.; Yunis-Aguinaga, Jefferson
    Objectives: The purpose of this study was to analyze the genome of the multidrug-resistant Citrobacter freundii strain CAPA023, which was obtained from diseased Arapaima gigas fry. The study focused on determining mobile genetic elements and genetic factors that contribute to antibiotic resistance and pathogenicity. Methods: Genomic DNA was sequenced using Illumina NovaSeq (2 × 150 bp) and assembled de novo using Shovill v1.1.0. Resistance genes, virulence factors, plasmids, and mobile elements were identified using ResFinder, CARD, PlasmidFinder, MobileFinder, PathogenFinder, and VFDB. Results: The 5,059,550 bp draft genome (60 contigs, 51.5% GC) revealed resistance genes for various antibiotic classes, efflux pumps, IncFIB(K) and Col440I plasmids, insertion sequences, and multiple virulence genes. Conclusion: Considering that this bacterium was found in diseased fish, it is possible that C. freundii plays an important role in the spread of virulence factors and antibiotic resistance in aquaculture environments. This highlights the importance of genomic surveillance in Amazonian aquaculture. © 2025 Elsevier B.V., All rights reserved.
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    Histological lesions by monogeneans in gills of Piaractus brachypomus farmed in semi-intensive systems from Peru
    (Brazilian Journal of Veterinary Pathology, 2024) Torres-Lozano, Karel; Cabrera-Soregui, Milagros; Garcia-Candela, Enrique; Cuadros-Cuya, Martín; Cubas-Rengifo, Andrés; Guzmán, Víctor Humberto Puicón Niño De; Mesias, Fernando
    Piaractus brachypomus Cuvier, 1818, is Peru’s most produced Amazonian fish; however, little is known about histological lesions that monogeneans produce in their gills. When examining 40 juveniles of P. brachypomus from two commercial fish farms, Anacanthorus penilabiatus (Boeger et al., 1995) and Mymarothecium viatorum (Boeger et al., 2002) were found, with a total monogenean prevalence of 100%, with a mean intensity and mean abundance of 225.5 parasites/fish for both species. At the level of the gill tissue, lesions identified were dilation and congestion of the central vein and hyperplasia of the secondary lamella with eosinophilic and lymphocytic infiltration. The histopathological alterations caused by infection of A. penilabiatus and M. viatorum are reported for the first timein cultured P. brachypomus in the Peruvian Amazon. These findings indicate the need to improve good practices and biosafety in producing this fish to prevent or control the impact of these monogeneans.
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    Molecular identification of Mymarothecium viatorum and Anacanthorus penilabiatus in extensive native fish farming systems of the Peruvian Amazon
    (Elsevier BV, 2024-9) Cabrera-Soregui, Milagros; Torres-Lozano, Karel; Garcia-Candela, Enrique; Mesias-Valle, Fernando; Martinez-Rojas, Rosa; Cruz-Neyra, Lidia; Mondragón-Martínez, Aarón; Ebert, Mariana Bertholdi; Da Silva, Reinaldo José; Cala-Delgado, Daniel Leonardo
    Piaractus brachypomus (Pacú) is the main native fish species cultivated in Peru and holds great potential for growth in aquaculture from the Peruvian Amazon. Between October 2021 and January 2022 in two fish producing farms in the Amazon region of San Martín in Peru, P. brachypomus individuals were examined for parasite evaluation. A total of 6366 monogeneans were isolated from the gills of 30 fish, revealing a prevalence of 100%, with an abundance and mean intensity of 212 parasites per fish. Monogeneans were morphologically identified as Mymarothecium viatorum and Anacanthorus penilabiatus. The genetic divergence in the 28S rDNA gene found among A. penilabiatus sequences was 0.1% and among Anacanthorus spp. it ranged from 0.9% to 7.5%. The genetic divergence found among the M. viatorum sequences was 0.3%. These finding represents the first molecular data of M. viatorum and A. penilabiatus in Peru using the 28S rDNA gene of these monogeneans. The new sequences obtained will contribute to future studies on the phylogenetic relationships among dactylogyrids. However, further research with a broader range of host-parasite samples and additional genetic markers is needed to clarify these relationships and provide stronger support for the phylogenetic positions. © 2024 Elsevier B.V., All rights reserved.